e coli c600  (ATCC)

Journal: Synthetic and Systems Biotechnology

Article Title: Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates

doi: 10.1016/j.synbio.2026.01.006

Figure Lengend Snippet: Investigation of xylose transport and PTS modification for succinate production. (A) Schematic representation of glucose and xylose transport routes in different E. coli strains, highlighting the key transporters and metabolic nodes influencing carbon flux; (B) Intracellular ATP levels in strains C600, MG1655, and BW25113 during aerobic growth on xylose; (C) Comparison of succinate and by-product accumulation between the parental strain C600 and engineered strain ESC2 under anaerobic conditions; (D) Fermentation performance of PTS-modified strain ESC3, showing sugar utilization, biomass generation, and succinate production; (E–F) Growth profiles of engineered ESC3 derivatives in defined medium with xylose (E) or glucose–xylose mixtures (F). All experimental data were performed in triplicate, and error bars represent the standard deviation. Statistical analysis was performed using a two-tailed Student's t -test (∗∗p < 0.01, ∗∗∗p < 0.001).

Article Snippet: In this study, we systematically engineered E. coli C600 (ATCC 23724) [ ], a strain with efficient and low-energy xylose transport, as the chassis for succinate production from lignocellulosic sugars.

Techniques: Modification, Comparison, Standard Deviation, Two Tailed Test

Journal: Synthetic and Systems Biotechnology

Article Title: Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates

doi: 10.1016/j.synbio.2026.01.006

Figure Lengend Snippet: Construction of a succinate-producing strain from C600. (A) Metabolic map illustrating targeted knockouts ( ldhA , pflB , ptsG , adhE and pta-ackA ) and expression/integration of pck to redirect flux toward succinate; (B) Two-stage fermentation scheme comprising aerobic growth using shaking flasks and followed by anaerobic production in serum bottles; (C–D) Succinate fermentation of six engineered strains cultured on xylose (C) or glucose–xylose mixtures (D). All experimental data were performed in triplicate, and error bars represent the standard deviation.

Article Snippet: In this study, we systematically engineered E. coli C600 (ATCC 23724) [ ], a strain with efficient and low-energy xylose transport, as the chassis for succinate production from lignocellulosic sugars.

Techniques: Expressing, Cell Culture, Standard Deviation

Journal: Synthetic and Systems Biotechnology

Article Title: Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates

doi: 10.1016/j.synbio.2026.01.006

Figure Lengend Snippet: Evaluation of exogenous xylose utilization pathways and library-based strain selection. (A) Schematic comparison of the endogenous XI pathway with the Dahms and Weimberg pathways; (B) Design of pathway plasmid libraries and RBS variants controlling expression of key genes for Dahms and Weimberg pathways. The Weimberg library plasmid carries XylA , XylX , and XylB from C. crescentus , while the Dahms library plasmid contains XylB from C. crescentus . The helper plasmid harbors xylC from C. crescentus and the endogenous yjhG from E. coli . RBS sequences were designed with 32 mutations, enabling gene expression levels ranging from 4 to 57,523 au; (C) Growth and succinate production of four representative ESC7 derivatives (ESC7-W1, ESC7-W2, ESC7-D1, ESC7-D2), which were randomly selected from the Weimberg (W1, W2) or Dahms (D1, D2) pathway libraries, compared with ESC6 (XI pathway); (D) Fermentation performance of the same four ESC7 clones carrying the helper plasmid (harboring XylC and yjhG ), compared with ESC6; (E) Validation of pathway combinations in the ESC6 background using the same four representative plasmids, integrating XI with Dahms/Weimberg routes and help plasmid; (F) Screening of library colonies identified six optimal variants, which were reconstructed in ESC6 and evaluated for succinate production from glucose–xylose mixtures. All experimental data were performed in triplicate, and error bars represent the standard deviation. Statistical analysis was performed using a two-tailed Student's t -test (∗∗∗ p < 0.001).

Article Snippet: In this study, we systematically engineered E. coli C600 (ATCC 23724) [ ], a strain with efficient and low-energy xylose transport, as the chassis for succinate production from lignocellulosic sugars.

Techniques: Selection, Comparison, Plasmid Preparation, Expressing, Gene Expression, Clone Assay, Biomarker Discovery, Standard Deviation, Two Tailed Test

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Antibacterial activities of the obtained peptides.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Concentration Assay

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Kinetics of changes in the E. coli ML35p cytoplasmic membrane permeability measured with the use of ONPG (OD 405 ) hydrolysis caused by the examined peptides. Peptide concentrations (μM) are presented within the dashed box. Control measurements were carried out without antimicrobial agents.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Membrane, Permeability, Control

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: MIC changes in the bacterial strain E. coli MDR CI 1057 exposed to resistance selection by the peptide HfBRI-25 (initial MIC value = 1 μM) and by the reference antibiotic polymyxin B (PmxB, initial MIC value = 0.125 μM). The experiment was performed in the Mueller–Hinton broth supplemented with 0.9% NaCl at 37 °C. Bacteria that grew at the highest concentrations of AMPs after the final passage (on the 21st day) were further passaged three times (“Post”) on drug-free agar plates before determining the final MIC value. No differences in MICs before and after 21 passages without antimicrobial agents were observed.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Selection, Bacteria

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Stability in blood serum and in vivo efficiency of the peptide HfBRI-25. ( A ) Stability of HfBRI-25 in serum. The peptides were incubated at 37 °C in 25% buffered human serum and analyzed by RP-HPLC. * The ratio (a/b) of the peptide forms was estimated according to MALDI-TOF MS data. These data are presented as the mean ± SD of two independent experiments. ( B ) Survival rates of BALB/c mice ( n = 5) infected intraperitoneally with E. coli ATCC 25922 (10 6 bacteria in the presence of 2.5% mucin). Ciprofloxacin and saline were used as positive and negative controls, respectively. Health status of the examined mice was checked once a day for 7 days after infection.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: In Vivo, Incubation, Infection, Bacteria, Saline

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Antibacterial activity of HfBRI-25 against E. coli strains.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Activity Assay, Concentration Assay

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Bacterial strains used in this study.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Cloning, Expressing

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Impact of HfBRI-25 and conventional antibiotic ciprofloxacin on bacterial cell viability inside the biofilm formed by E. coli K12 SBS 1936. *** p < 0.001 (significantly different compared to the control).

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Control