e coli c600  (ATCC)

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Antibacterial activities of the obtained peptides.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Concentration Assay

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Kinetics of changes in the E. coli ML35p cytoplasmic membrane permeability measured with the use of ONPG (OD 405 ) hydrolysis caused by the examined peptides. Peptide concentrations (μM) are presented within the dashed box. Control measurements were carried out without antimicrobial agents.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Membrane, Permeability, Control

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: MIC changes in the bacterial strain E. coli MDR CI 1057 exposed to resistance selection by the peptide HfBRI-25 (initial MIC value = 1 μM) and by the reference antibiotic polymyxin B (PmxB, initial MIC value = 0.125 μM). The experiment was performed in the Mueller–Hinton broth supplemented with 0.9% NaCl at 37 °C. Bacteria that grew at the highest concentrations of AMPs after the final passage (on the 21st day) were further passaged three times (“Post”) on drug-free agar plates before determining the final MIC value. No differences in MICs before and after 21 passages without antimicrobial agents were observed.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Selection, Bacteria

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Stability in blood serum and in vivo efficiency of the peptide HfBRI-25. ( A ) Stability of HfBRI-25 in serum. The peptides were incubated at 37 °C in 25% buffered human serum and analyzed by RP-HPLC. * The ratio (a/b) of the peptide forms was estimated according to MALDI-TOF MS data. These data are presented as the mean ± SD of two independent experiments. ( B ) Survival rates of BALB/c mice ( n = 5) infected intraperitoneally with E. coli ATCC 25922 (10 6 bacteria in the presence of 2.5% mucin). Ciprofloxacin and saline were used as positive and negative controls, respectively. Health status of the examined mice was checked once a day for 7 days after infection.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: In Vivo, Incubation, Infection, Bacteria, Saline

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Antibacterial activity of HfBRI-25 against E. coli strains.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Activity Assay, Concentration Assay

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Bacterial strains used in this study.

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Cloning, Expressing

Journal: Marine Drugs

Article Title: Novel BRICHOS-Related Antimicrobial Peptides from the Marine Worm Heteromastus filiformis : Transcriptome Mining, Synthesis, Biological Activities, and Therapeutic Potential

doi: 10.3390/md21120639

Figure Lengend Snippet: Impact of HfBRI-25 and conventional antibiotic ciprofloxacin on bacterial cell viability inside the biofilm formed by E. coli K12 SBS 1936. *** p < 0.001 (significantly different compared to the control).

Article Snippet: E. coli ATCC 23724 , 0.5 , 1 , 2.

Techniques: Control